Journal of Stress Physiology & Biochemistry, Vol. 18 No. 2 2022, pp. 43-48 ISSN 1997-0838
Original Text Copyright (cc) 2021 by  Al-Daoude, Jawhar, Al-Shehadah, Shoaib and Arabi

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Hydrogen Peroxide and Phenylalanine Ammonia-lyase as Signalling Molecules in Barley Leaves Challenged with Cochliobolus sativus

Antonious Al-Daoude, Mohammed Jawhar, Eyad Al-Shehadah, Amina Shoaib and Mohammed Imad Eddin Arabi

1 Department of Molecular Biology and Biotechnology, AECS, P. O. Box 6091, Damascus, Syria.


Received November 6, 2021

Hydrogen peroxide (H2O2) and phenylalanine ammonia-lyase (PAL) have been reported as important signaling molecules during plant resistance against many fungal pathogens. In this study, the relative contributions of H2O2 and PAL were investigated at early time periods of barley infection with Cochliobolus sativus, the causal agent of spot blotch disease. H2O2 activity was observed in leaf tissues 24 hours post inoculation (hpi) and was accompanied with an increase in PAL expression in resistant and susceptible genotypes. However, the resistant genotype ‘Banteng’ contained higher levels of H2O2 and PAL, as compared with the susceptible one ‘WI 2291’. Results demonstrated that the cooperative function of H2O2 and PAL in barley responses to C. sativus appeared to be dependent on the plant genotype, and it is hypothesized that the peak of activity of PAL at 48h and 72 h, and the rapid increase in H2O2 24 h in resistant and susceptible genotypes are considered general defense responses.

Key words:    Hordeum vulgare, Cochliobolus sativus, H2O2, PAL expression, real time PCR

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