TY  - JOUR
AU  - Al-Daoude, Antonious
AU  - Jawhar, Mohammed
AU  - Al-Shehadah, Eyad
AU  - Shoaib, Amina
AU  - Arabi, Mohammed Imad Eddin
T1  - Hydrogen Peroxide and Phenylalanine Ammonia-lyase as Signalling Molecules in Barley Leaves Challenged with Cochliobolus sativus
JO  - Journal of Stress Physiology & Biochemistry
Y1  - 2022/june
VL  - 18
IS  - 2
SP  - 43
EP  - 48
UR  - http://www.jspb.ru/issues/2022/N2/JSPB_2022_2_43-48.pdf
KW  - Hordeum vulgare
KW  - Cochliobolus sativus
KW  - H2O2
KW  - PAL expression
KW  - real time PCR
U1  - 1997-0838
N2  - Hydrogen peroxide (H2O2) and phenylalanine ammonia-lyase (PAL) have been reported as important signaling molecules during plant resistance against many fungal pathogens. In this study, the relative contributions of H2O2 and PAL were investigated at early time periods of barley infection with Cochliobolus sativus, the causal agent of spot blotch disease. H2O2 activity was observed in leaf tissues 24 hours post inoculation (hpi) and was accompanied with an increase in PAL expression in resistant and susceptible genotypes. However, the resistant genotype ‘Banteng’ contained higher levels of H2O2 and PAL, as compared with the susceptible one ‘WI 2291’. Results demonstrated that the cooperative function of H2O2 and PAL in barley responses to C. sativus appeared to be dependent on the plant genotype, and it is hypothesized that the peak of activity of PAL at 48h and 72 h, and the rapid increase in H2O2 24 h in resistant and susceptible genotypes are considered general defense responses.
ER  -