Journal of Stress Physiology & Biochemistry, Vol. 16 No. 4 2020, pp. 82-94  ISSN 1997-0838
Original Text Copyright (cc) 2020 by  Jawdat, Abu Kassem, Saleh, Aljapawe, Ikhtiar and Al-Safadi



ORIGINAL ARTICLE
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Priming of Long-Term Stored Cotton Seeds Using Combined UV-A, B and C Radiation and Its Influence on Germination

Dana Jawdat1*, Issam Abu Kassem2, Aghyad Saleh1, Abdulmunim Aljapawe1, Adnan Ikhtiar1, and Bassam Al-Safadi1 

1 Department of Molecular Biology and Biotechnology, Atomic Energy Commission of Syria (AECS), Damascus, P. O. Box 6091
2 Department of Protection and Safety, Atomic Energy Commission of Syria (AECS), Damascus, P. O. Box 6091

*E-Mail: ascientific@aec.org.sy

Received June 16, 2020

Seeds vigour and uniform germination across diverse environmental conditions is a primary objective in agriculture. Moisture and temperature are key factors affecting cotton seed quality during storage, where maintenance is rather difficult during long-term storage. To investigate the potential influence of UV radiation on enhancing cotton seed vigour after long-term storage, Deir Al-Zour 22 cotton variety was selected due to its reduced-vigour by time and its low germination rates. Germination rates of cotton seeds exposed to combined UV-A, B and C irradiation for different periods of time (4, 8, 12, and 16 min) were enhanced compared to non-irradiated seeds. Data showed improved growth of generated seedlings on PEG and NaCl supplemented media. Results showed no major changes on the expression of GA3ox1 gene, whereas, two stress-related genes DEH and VPP were temporarily activated after treatment with UV-irradiation supporting their function as scavenging and accumulating factors of ROS, a typical by-product of the photo-excitation under UV. Our results suggest the possibility of using combined UV-A, B and C radiation as a physical priming agent of cotton seeds to induce plant vigor and enhance germination under stress conditions without affecting normal growth.

Key words:   Ultraviolet; priming; cotton; gene expression; flow cytometry

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