TY  - JOUR
AU  - Jawdat, Dana
AU  - Kassem, Issam Abu
AU  - Saleh, Aghyad
AU  - Aljapawe, Abdulmunim
AU  - Ikhtiar, Adnan
AU  - Al-Safadi, Bassam
T1  - Priming of Long-Term Stored Cotton Seeds Using Combined UV-A, B and C Radiation and Its Influence on Germination
JO  - Journal of Stress Physiology & Biochemistry
Y1  - 2020
VL  - 16
IS  - 4
SP  - 82
EP  - 94
UR  - http://www.jspb.ru/issues/2020/N4/JSPB_2020_4_82-94.pdf

KW  - Ultraviolet; priming; cotton; gene expression; flow cytometry

U1  - 82-94




N2  - Seeds vigour and uniform germination across diverse environmental conditions is a primary objective in agriculture. Moisture and temperature are key factors affecting cotton seed quality during storage, where maintenance is rather difficult during long-term storage. To investigate the potential influence of UV radiation on enhancing cotton seed vigour after long-term storage, Deir Al-Zour 22 cotton variety was selected due to its reduced-vigour by time and its low germination rates. Germination rates of cotton seeds exposed to combined UV-A, B and C irradiation for different periods of time (4, 8, 12, and 16 min) were enhanced compared to non-irradiated seeds. Data showed improved growth of generated seedlings on PEG and NaCl supplemented media. Results showed no major changes on the expression of GA3ox1 gene, whereas, two stress-related genes DEH and VPP were temporarily activated after treatment with UV-irradiation supporting their function as scavenging and accumulating factors of ROS, a typical by-product of the photo-excitation under UV. Our results suggest the possibility of using combined UV-A, B and C radiation as a physical priming agent of cotton seeds to induce plant vigor and enhance germination under stress conditions without affecting normal growth.
ER  -