Journal of Stress Physiology & Biochemistry, Vol. 10 No. 1 2014, pp. 37-44 ISSN 1997-0838
Original Text Copyright (cc) 2014 by   Mohamed, Aly, Omar



ORIGINAL ARTICLE
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Use of Phenols, Peroxidase and Polyphenoloxidase of Seed to Quantify Resistance of Cotton Genotypes to Damping-off Incited by Fusarium oxysporum

Heba I. Mohamed 1*, Aly A. Aly 2 and Moawad R. Omar 2

1     Department of Biological and Geological Sciences, Faculty of Education, Ain Shams University, Cairo, Egypt
2     Plant Pathology Research Institute, Agricultural Research Center, Giza, Egypt

*E-Mail: hebaibrahim79@yahoo.com

Received August 12, 2013

A greenhouse test was conducted in 2011 and 2012 growing seasons at Giza Agricultural Research Station to evaluate the reaction of six cotton genotypes to damping-off incited by Fusarium oxysporum. Damping-off incidence on the genotypes ranged from 70-88%. In general, the genotypes could be divided into highly susceptible, susceptible, and moderately susceptible. Data for damping-off incidence and level or activity of some biochemical components (phenols, peroxidase, and polyphenoloxidase) were entered into a computerized linear regression analysis. The analysis contrasted seven predictive models by using the biochemical components, singly or in combination, as biochemical predictors. It was evident that models nos. 2 and 6 were the best models for predicting incidence of damping-off. The superiority of these models was attributed to their high Rē values (0.748 and 0.902, respectively) and the significance of their F. values (P = 0.026 and P = 0.031, respectively). The results of the present study suggest that peroxidase alone or both peroxidase and polyphenoloxidase, which may or may not parts of damping-off resistance mechanisms, can be used as biochemical markers to predict resistance to damping-off incited by F. oxysporum.


Key words:    Fusarium oxysporum, Gossypium barbadense, peroxidase, phenol, polyphenol oxidase

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