Journal of Stress Physiology &
Biochemistry, Vol. 9 No. 4
2013, pp. 352-361 ISSN 1997-0838
Original Text Copyright (cc) 2013 by Katyshev, Chernikova, Subota,
Tarasenko, Klimenko, Shmakov, Grebnev, Konstantinov
ORIGINAL ARTICLE
Data source : Google Scholar
QueryDate : 2016-12-24
Cites : 0 + 0
Characterization of Maize
Superoxide Dismutase Gene Family: Identification of Two Novel
Transcripts of Chloroplast SODs and Analysis of Potential
DNA-protective Function of Mitochondrial MnSOD
Siberian Institute of Plant Physiology and
Biochemistry, Siberian Branch of RAS, Irkutsk, Russia
*E-Mail: alex@sifibr.irk.ru
Received August 29, 2013
The families of genes encoding such
key antioxidant enzymes as superoxide dismutases usually have
complex structure. They consist of
genes encoding enzymes which differ
by their metal cofactor, origin and subcellular
localization. The maize SOD family consisting of multiple genes is one
of the most interesting among the characterized plant SOD gene
families. We re-investigated the structure of maize SOD gene family
using different approaches. We revealed two novel gene transcripts
encoding chloroplast Fe- and Cu/Zn-SOD. This finding indicates that the
reactive oxygen species (ROS) detoxication system in maize chloroplasts
has more complicated organization than it was considered earlier. At
the same time it appeared that the number of maize genes encoding
mitochondrial Mn-SODs is less than it was supposed. Using the
recombinant proteins we showed that the functioning
of MnSOD enzymes is posttranslationally
regulated by phosphorylation/dephosphorylation.
Possible involvement of Mn-SOD in
the protection of mitochondrial DNA from oxidative damage
is investigated.
