TY  - JOUR
AU  - Katyshev, A.I.
AU  - Chernikova, V.V.
AU  - Subota, I.Y.
AU  - Tarasenko, V.I.
AU  - Klimenko, E.S.
AU  - Shmakov, V.N.
AU  - Grebnev, P.A.
AU  - Konstantinov, Y.M.
T1  - Characterization of Maize Superoxide Dismutase Gene Family: Identification of Two Novel Transcripts of Chloroplast SODs and Analysis of Potential DNA-protective Function of Mitochondrial MnSOD
JO  - Journal of Stress Physiology & Biochemistry
Y1  - 2013
VL  - 9
IS  - 4
SP  - 352
EP  - 361
UR  - http://www.jspb.ru/issues/2013/N4/JSPB_2013_4_352-361.pdf

KW  - reactive oxygen species
KW  - superoxide dismutase
KW  - chloroplasts
KW  - mitochondria




N1  - Owner: Алексей
N1  - Added to JabRef: 2013.10.14
N2  - The families of genes encoding such key antioxidant enzymes as superoxide dismutases usually have  complex  structure.  They  consist  of  genes  encoding  enzymes  which  differ  by  their  metal cofactor, origin and subcellular localization. The maize SOD family consisting of multiple genes is one of the most interesting among the characterized plant SOD gene families. We re-investigated the structure of maize SOD gene family using different approaches. We revealed two novel gene transcripts encoding chloroplast Fe- and Cu/Zn-SOD. This finding indicates that the reactive oxygen species (ROS) detoxication system in maize chloroplasts has more complicated organization than it was considered earlier. At the same time it appeared that the number of maize genes encoding mitochondrial Mn-SODs is less than it was supposed. Using the recombinant proteins we showed that  the  functioning  of  MnSOD  enzymes  is  posttranslationally  regulated  by phosphorylation/dephosphorylation.  Possible  involvement  of  Mn-SOD  in  the  protection  of mitochondrial DNA from oxidative damage is investigated.
ER  -