ORIGINAL ARTICLE

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Morphological and molecular variation of five rice varieties to Ultra Violet-B radiation stress

A. John De Britto *, R. Mary Sujin and Steena Roshan Sebastian

Plant Molecular Biology Research Unit, St.Xavier's College, (Autonomous), Palayamkottai. Tamilnadu, India.
*Email: bjohnde@yahoo.co.in

Received April 30, 2011

India is one of the major rice producing countries. Rice cultivation is affected by several factors such as climate, soil pollution, UV radiation etc. The present study is an attempt made to understand the morphological variation and molecular variation through SDS-PAGE in five different rice varieties induced with UV-B stress.

Five varieties of rice were irradiated with UV-B for 10, 20 and 30 min and a set was maintained as control for every variety. Morphological variations were estimated using morphometric analysis that showed significant variation in stressed and controlled sets. The leaf protein was separated through SDS-PAGE gel electrophoresis and molecular weight markers were used to calculate the molecular weight of the proteins.

UV treated varieties had the lowest record based on the banding pattern than the control. These results show that the UV radiation could induce both phenotypic and genotypic changes in rice.

Key words: Morphological parameters, Molecular variation, Oryza sativa L., SDS-PAGE, UV stress.