Journal of Stress Physiology & Biochemistry, Vol. 19, No. 4, 2023, pp. 139-147       ISSN 1997-0838
Original Text Copyright (cc) 2023 by  Kavitha and Nandagopalan



ORIGINAL ARTICLE
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Quantitative Phytochemical Analysis on Leaf Extract of Calanthe masuca (D.Don) Lindl., an Endangered Medicinal Plant (Orchidaceae)

Duraisamy Kavitha1* and Veeraiyan Nandagopalan2 

1
PG and Research Department of Botany, National College, Affiliated to Bharathidasan University, Tiruchirappalli-620001, Tamil Nadu, India.
2 Associate Professor, Department of Botany, National College, Affiliated to Bharathidasan University, Tiruchirappalli-620001, Tamil Nadu, India

*E-Mail: kavithaabarna123@gmail.com


Received August 14, 2023


Calanthe masuca (D.Don) Lindl plant leaves have medicinal properties. It is an endangered and terrestrial orchid. They are distributed in evergreen forest; plant collection is very difficult for researches. That’s why very rare because of group of plants not widely represent in the forest. The present study has aimed to evaluate the phytochemical analysis using three different solvents Methanol, Aqueous and Petroleum Ether. The secondary metabolites have estimated into phenols, flavonoids, alkaloids, tannins, saponins, steroids and terpenoids. The result showed that the methanol leaf extract shows as a high concentration. Aqueous extract showed moderate concentration and Petroleum ether extract showed low concentration. Methanol extract contained secondary metabolites such as the total phenol (31.500.22 mg/g) and tannins (24.600.02 mg/g) as mg, alkaloids (54.400.33 mg/g) as mg, flavonoids (73.200.62 mg/g) as mg, saponins (35.000.05 mg/g), Steroid (60.950.57 mg/g) and Terpenoids (14.000.26 mg/g). The Calanthe masuca is a medicinal plant based on the secondary metabolites presence of phenols, flavonoids, alkaloids, tannins, saponins, steroids and terpenoids. They are used as a source of potentials therapeutic medicinal values and curing various infectious diseases.

Key words:    Medicinal plant, therapeutic drugs, secondary metabolites, Orchidaceae

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