Diabetes mellitus is a group of chronic metabolic disease characterized by elevated level of glucose in blood. It is expected that by the year 2030, the number of diabetes will rise up to 439 million, and greater than 90% have type 2 diabetes. In vitro antidiabetic potential of ethanol extract of Parmelia perlata Ach. (EEPP) was determined by evaluating the inhibitory enzymes of α- amylase, sucrase and α- glucosidase. Glucose adsorption, diffusion, glucose uptake by yeast cell and non-enzymatic glycation parameters were also studied. Cytotoxic assay and glucose uptake assay were carried out in 3T3L1 cell line. Ethanolic extract of Parmelia perlata Ach. showed dose dependent effect on inhibition of sucrase, α- amylase and α- glucosidase. Results for glucose bounding capacity, glucose diffusion, glucose uptake by yeast cell and non- enzymatic glycation were also promising. In vitro studies in 3T3L1 preadipocyte cell line showed effective non cytotoxicity and enhanced glucose uptake. From the data of the experiment it was evident that EEPP might be a potent natural drug source for treating diabetes mellitus.
Key words: α-amylase, α-glucosidase, glucose adsorption, glucose diffusion, Parmelia perlata