Background: The current study focuses on the participation of total phenolic content (TPC), total flavonoid content (TFC) as well as antioxidant activity in germinating soybean seeds under abiotic stress.
The context and purpose of the study: For this study, soybean seeds were subjected to three abiotic stresses namely drought, flood, and salinity for 3 days.
Method: The estimation of TPC and TFC was performed by FTIR and spectrophotometric method. The spectrophotometric estimation of TPC and TFC was performed by Folin Ciocalteu and Aluminium Chloride method respectively.
Results: The highest TPC (68.78 µg gallic acid/ml) and TFC content (6.23 µg quercetin/ml) found in drought treated seeds for one day. In terms of the DPPH scavenging assay, the highest (67.63%) and lowest (46.83%) percentage of inhibition was observed on the1st and 3rd day of salinity stress respectively. Results of the current study also showed a strong positive correlation between TPC and TFC analyzed by FTIR and Spectral data.
Conclusion: The study supports the role of phenolic and flavonoid in germinating soybean seeds under abiotic stress.
Key words: Abiotic stress, Phenolic, Flavonoid, FTIR, Soybean (Glycine max).