Journal of Stress Physiology & Biochemistry, Vol. 19 No. 1 2023, pp. 141-153   ISSN 1997-0838
Original Text Copyright (cc) 2022 by Sirsat, Nadaf and Hivrale



ORIGINAL ARTICLE
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Differential hikes in phenolic and flavonoid compounds in germinating soybean (Glycine max) seeds under abiotic stresses

Ashwini K. Sirsat, Heena L. Nadaf, and Vandana K. Hivrale*

1 Department of Biochemistry, Dr. Babasaheb Ambedkar Marathwada University, Aurangabad, Maharashtra, India

*E-Mail: vkhbiochem@gmail.com

Received October 22, 2022

Background: The current study focuses on the participation of total phenolic content (TPC), total flavonoid content (TFC) as well as antioxidant activity in germinating soybean seeds under abiotic stress.

The context and purpose of the study: For this study, soybean seeds were subjected to three abiotic stresses namely drought, flood, and salinity for 3 days.

Method: The estimation of TPC and TFC was performed by FTIR and spectrophotometric method. The spectrophotometric estimation of TPC and TFC was performed by Folin Ciocalteu and Aluminium Chloride method respectively.

Results: The highest TPC (68.78 g gallic acid/ml) and TFC content (6.23 g quercetin/ml) found in drought treated seeds for one day. In terms of the DPPH scavenging assay, the highest (67.63%) and lowest (46.83%) percentage of inhibition was observed on the1st and 3rd day of salinity stress respectively. Results of the current study also showed a strong positive correlation between TPC and TFC analyzed by FTIR and Spectral data.

Conclusion: The study supports the role of phenolic and flavonoid in germinating soybean seeds under abiotic stress.

Key words:    Abiotic stress, Phenolic, Flavonoid, FTIR, Soybean (Glycine max).

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