Journal of Stress Physiology & Biochemistry, Vol. 18 No. 2 2022, pp. 93-95   ISSN 1997-0838
Original Text Copyright (cc) 2022 by  Nassar, Sharif and Hammad

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A Modified d-ROMs Method for Determination of Lipid Peroxidation

Muammar A Y Nassar, Saleh H Sharif, Khalda M A Hammad
1 The Biomedical Research Laboratory (BRL) group, Faculty of Medicine and Health Sciences, University of Dongola, Dongola, Sudan.


Received January 16, 2022

The need for simple and low costing and less time consuming methods for the determination of lipid peroxides and oxidative stress is urgent because oxidative stress is still unable to become a routine laboratory biomarker due to the sophisticated procedures and costing, a matter that made oxidative stress a research only biomarker, despite its crucial value as a risk factor and pathologic status, for many inflammatory and metabolic diseases. This work aimed at developing a modified rapid method to determine serum lipid peroxides at wavelength 492 nm and reference wavelength 630 nm. The results showed linear responses with increasing concentration and that the cheap N,N-DM-P-Phenylenediamine diHCl can be used as a chromogen under saving conditions of pH, temperatures and volume of sample.

Key words:    determination of lipid peroxides, modified rapid method

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