TY - JOUR AU - Rakhra, Gurmeen AU - Sharma, Arun Dev T1 - Role of Boiling Soluble Protein Disulphide Isomerase (BsPDI) under Drought Stress in Divergent Cultivars of Wheat JO - Journal of Stress Physiology & Biochemistry Y1 - 2021/december VL - 17 IS - 4 SP - 77 EP - 84 UR - http://www.jspb.ru/issues/2021/N4/JSPB_2021_4_77-84.pdf KW - Boiling soluble protein disulphide isomerase KW - chaperones KW - Days post Anthesis KW - drought stress KW - Triticum aestivum. U1 - 1997-0838 N2 - Background: To protect them from water stress induced-ROS- mediated protein unfolding and aggregation, plants are equipped with a wide range of antioxidant redox molecular chaperonic proteins like Protein disulphide Isomerase (PDI) (E.C.5.3.4.1). These are a diverse group of proteins that in vivo bind to misfolded or unfolded proteins and play an important role to form specific three dimensional conformation of the functional proteins. In addition, stress conditions induce altered and intensified PDI expression in plant cell, thereby highlighting the role of these proteins under abiotic stress conditions. The context and purpose of the study; The main objective of the study was to determine drought stress- induced changes in the modulation of the boiling soluble protein disulphide isomerase (BsPDI) in response to drought at two different developmental stages 38 Days Post Anthesis (DPA) and 52 DPA in Triticum aestivum. Results, the main findings; A temporal regulation of BsPDI accumulation in a cultivar dependent manner was observed under control and drought stress. SDS-PAGE and Western blot analysis revealed strong induction of BsPDI17 under drought conditions only in the tolerant cv. PBW 527 at 38 DPA. Contrary to this, unchanged BsPDI17 accumulation was detected in the sensitive cv. PBW 343 at 38 DPA under drought. However, at 52 DPA, there was a marked decline in BsPDI17 accumulation in the sensitive cv. PBW 621 under stress conditions. Conclusions, brief summary and potential implications: Based upon our results, significance of BsPDI in the wheat cultivars differing in drought resistance during stress conditions is discussed. ER -