Journal of Stress Physiology & Biochemistry, Vol. 16 No. 4 2020, pp. 70-81  ISSN 1997-0838
Original Text Copyright (cc) 2020 by   Vikrant, Kothai and Roselin Roobavathi 

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Evaluation of Salinity Stress Effects on Seed Germination and Seedling Growth and Estimation of Protein Contents in Kodo Millet (Paspalum scrobiculatum L.)

Vikrant*, N. Kothai, and M. Roselin Roobavathi

  Department of Botany, Kanchi Mamunivar Government Institute for Postgraduate Studies and Research (Autonomous), Puducherry- 605 008, India.


Received June 14, 2020

Cereals in general and millets in particular have been identified as major food crops globally and increase in crop production is required to meet the demands of the ever increasing global population. However, continuous climatic variations or abiotic stresses have led the drastic reduction in food grain yields. In view of affects of abiotic stresses on food crops plants, present study was undertaken to analyze the comparative responses of salinity stresses induced by various concentrations of NaCl (50mM, 100mM, 250mM and 500mM) and sea water (5%, 10%, 15%, 20%, 25%, 50%, 75% and 100%) during seed germination and seedling growth under ex-vitro conditions in kodo millet (Paspalum scrobiculatum L.). After 6-days and 12-days of salinity stress treatments, observations were recorded and after 12-days of treatments, results reveal that salinity stress caused by NaCl-salt concentration (250mM) proves to be very lethal causing strong seed germination and therefore, the mean germination frequency (33% 0.530 was recorded while further increase in NaCl concentration (500mM) was found to be fully toxic and seed germination frequency was obtained as zero in comparison to control experiment (94% 0.35). Similarly, in case of salinity stress induced by sea water treatments, 25% of the sea water concentration was found to cause strong promotion instead of inhibition and (71% 0.17) of the seeds could exhibit promotion in germination frequency and further increase in sea water concentration (50%) and above was turned out to be fully toxic. Furthermore, during biochemical studies, protein contents in the tissues growing under NaCl-salinity stresses at (50mM, 100mM and 250mM) were estimated and significantly it was found to decline with the increase in concentration of NaCl-salt stress solutions. After 12th days of treatments, protein contents were found to be minimum (198.2 mg/g) in the tissues that were growing in high concentration of NaCl (250mM) solution than the control solution (476.4mg/g) grown tissues.  

Key words:   Abiotic Stress, Kodo Millet, Protein, Salinity, Sea Water, Seed Germination, Seedling

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