Journal of Stress Physiology & Biochemistry, Vol. 16 No. 3 2020, pp. 5-13 ISSN 1997-0838
Original Text Copyright (cc) 2020 by  Nandini and Vikas

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Development and Validation of a Simple HPLC PDA Method for the Simultaneous Analysis of 13- Docosenamide, Squalene and n-Tetracosanol-1 from the Leaf Extracts of Wagatea spicata

Girish Nandini and Vaidya Vikas

Ramnarain Ruia Autonomous College, L. Nappo Road, Dadar East, Matunga, Mumbai, Maharashtra 400019, (India)


Received February 29, 2020

Wagatea spicata (Dalzell) Wight [Moullava spicata (Dalzell) Nicolson] exhibits a diverse concentration of biologically active constituents such as Lupeol, Bergenin, Stigmasterol, Friedelin, n-Hexadecanoic acid, Palmitic acid, Gamma sitosterol,13docosenamide, squalene and n-Tetracosanol-1.
Present work focuses on development and validation of a precise, accurate and reproducible HPLC method for simultaneous quantification of three pharmacologically active phytochemical markers 13docosenamide, squalene and n-Tetracosanol-1 from the aerial parts of Wagatea spicata

Key words:    HPLC, 13-docosenamide, squalene, n-Tetracosanol-1, simultaneous, method development, method validation

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