Journal of Stress Physiology & Biochemistry, Vol. 15 No. 2 2019, pp. 37-44 ISSN 1997-0838
Original Text Copyright (cc) 2019 by  Nagaraju and Venkata Rathnamma

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Determination of Pesticide Residues in Freshwater Fish Tissues by Gas Liquid Chromatography

B. Nagaraju1 and V. Venkata Rathnamma2

1 Department of Biochemistry, Acharya Nagarjuna University, Guntur-522510, A.P, India.
2 Department of Zoology, Acharya Nagarjuna University, Guntur-522510, A.P, India.


Received March 25, 2019

In this present study, identification and quantification pesticide residues in freshwater fish Labeo rohita. Gas Liquid chromatography (GLC) method is successfully developed for the determination of profenofos residues. The fish were exposed to 1/10th sublethal concentration of profenofos 10 g/L-1 for 15 days. After the exposure fish was sacrifice and organs like Gil, Liver, Kidney, and Muscle were separated, the fish samples were extracted with n-Hexane, cleaned up and purified through solid-phase extraction method. The residues are in the following order, Gill > Muscle > Kidney > Liver, these results suggest that prolonged exposure to sublethal concentrations of profenofos in Labeo rohita leads to increased accumulation of pesticide residues in tissues.

Key words:    GLC, Profenofos, Liver, Pesticide, Residue

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