Haemolymph of Cold-Adapted Baikal Endemic Amphipods as a Promising Source for Screening of Novel Natural Products

Axenov-Gribanov, D.V.; Protasov, E.S.; Voytsekhovskaya, I.V.; Shatilina, Z.M.; Rzhechitskiy, Y.A.; Dimova, M.D.; Kostka, D.V.; Lubyaga, Y.A.; Timofeyev, M.A.

Journal of Stress Physiology & Biochemistry, Vol. 13 No. 4 2017, pp. 74-80 ISSN 1997-0838
Original Text Copyright (cc) 2017 by Axenov-Gribanov, Protasov, Voytsekhovskaya, Shatilina, Rzhechitskiy, Dimova, Kostka, Lubyaga and Timofeyev

ORIGINAL ARTICLE

Haemolymph of Cold-Adapted Baikal Endemic Amphipods as a Promising Source for Screening of Novel Natural Products

Axenov-Gribanov D.V. ^1,2*, Protasov E.S. ¹, Voytsekhovskaya I.V. ¹, Shatilina Z.M. ^1,2, Rzhechitskiy Y.A. ¹, Dimova M.D. ¹, Kostka D.V. ¹, Lubyaga Y.A. ^1,2, Timofeyev M.A. ¹

¹ Institute of Biology at Irkutsk State University, Irkutsk, 3 Lenin str., Russia
² Baikal Research Centre, Irkutsk, 21 Lenin str., Russia

*E-Mail: denis.axengri@gmail.com

Received September 2, 2017

The aim of this study was to assess the possibility to use Baikal endemic amphipods as a source for screening of novel natural products. Analysis of amphipod’s E. verrucosus hemolymph by HPLC-MS was performed for the first time. A number of natural products with new molecular mass for Baikal endemic macroinvertebrates were found. We found a compound as chrysin by comparison of MS¹, MS² profiles and molecular mass of natural products registered in Dictionary of Natural Products and MZ-cloud databases. Also, we found new compound with mass 493.80277 Da, circulating in the amphipod’s hemolymph.

Key words: amphipoda, hemolymph, dereplication, Baikal, natural products