Journal of Stress Physiology & Biochemistry, Vol. 13 No. 2 2017, pp. 45-54 ISSN 1997-0838
Original Text Copyright (cc) 2017 by  Romanenko, Romanenko, Babenko and Kosakivska

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Peculiarities of the Growth and Photosynthetic Pigments Content in Algaeculture of Acutodesmus dimorphus (Tupin) P.M. Tsarenko under Salt and Acetate Stresses

K.O. Romanenko1*, P.O. Romanenko2, L.M. Babenko1, I.V. Kosakivska1

1 M.G. Kholodny Institute of Botany of the National Academy of Sciences of Ukraine, 2 Tereshchenkivska str., 01601, Kyiv-1, Ukraine
2 Taras Shevchenko National University of Kyiv, Educational and Scientific Centre “Institute of Biology and Medicine”, Volodymyrska str., 64/13, 03022, Kyiv, Ukraine


Received March 29, 2017

The objective of this research was to investigate the effect of salt (NaCl) and acetate (NaC2H3O2) stresses on the pattern of biomass accumulation and photosynthetic pigments content in algae culture of the freshwater green alga Acutodesmus dimorphus (Turpin) P.M. Tsarenko IBASU-A 251. We demonstrated that sodium chloride introduced in a culture medium at various concentrations caused a gradual reduction of the microalga biomass whose maximum was recorded at the salt concentration of 1.5%. Addition of sodium acetate at the same concentrations led, on the contrary, to a sharp (more than two times) decrease of A. dimorphus biomass. On the 18th day of culturing under salt stress conditions the chlorophyll a content decreased 1.5-2.5 times, the chlorophyll b content – 1.3-1.7 times, while the carotenoids quantity increased 1.2-1.6 times. Under acetate stress conditions the chlorophyll a content decreased 2-3 times, that of chlorophyll b – 1.7 – 1.8 times whereas the carotenoids quantity increased within the range of 1.4-1.8 times. Increase in chemical stressors concentrations involved some reduction of the chlorophylls а/b ratio and total chlorophylls а+b content, and at the same time, the ratio carotenoids/a+b increased. Sodium acetate appeared to be a more powerful inducer of carotinogenesis than sodium chloride. The highest quantity of carotenoids was registered at 1% and 1.5% concentrations of NaC2H3O2 in the culture medium and 0.75% concentration of NaCl. The results obtained enable to regard Acutodesmus dimorphus (Turpin) P.M. Tsarenko IBASU-A 251 as an active carotenoids producer to be later applied in studies of hypersynthesis of individual classes of this pigment.

Key words:   Acutodesmus dimorphus, carotenoids, chlorophyll a, chlorophyll b, sodium acetate, sodium chloride

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