Journal of Stress Physiology &
Biochemistry, Vol. 12 No. 4 2016, pp. 91-95 ISSN 1997-0838
Original Text Copyright (cc) 2016 by Borvinskaya, Gurkov, Shchapova,
Baduev, Belousova, Meglinski and Timofeyev
ORIGINAL
ARTICLE
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Data source : Google Scholar
QueryDate : 2016-12-24
Cites : 0
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Repetitive Measurements of
Physiological pH by Implantable Optical Sensors in Muscles of adult Danio rerio: Preliminary Results
E.V. Borvinskaya1,2*,
A.N. Gurkov1,3, E.P. Shchapova1, B.K. Baduev1,3,
I.A. Belousova1,4, I.V. Meglinski1,5,
M.A. Timofeyev1
1
Institute of Biology at Irkutsk State University, 664003, Irkutsk,
Lenin str., 3, Russia
2 Institute of Biology at Karelian Research Centre of
Russian Academy of Sciences, 185035, Petrozavodsk, Pushkinskaya str.,
11, Russia
3 Baikal Research Centre, 664003, Irkutsk, Lenin str., 21,
Russia
4 Institute of Systematics and Ecology of Animals, Siberian
Branch of the Russian Academy of Sciences, 630091, Novosibirsk, Frunze
str. 11, Russia
5 University of Oulu, 90570, Oulu, Erkki
Koiso-Kanttilankatu, 3, Finland
*E-Mail:katsu@inbox.ru
Received
November 2, 2016
Encapsulated optical sensors are
promising tool for measurements of physiological parameters inside
living organisms. In the present study we tested the possibility to
apply encapsulated fluorescent sensors to measure pH in muscles of
adult Danio rerio. Right
after injection, the sensors detect slightly acidic pH, while after 3 h
pH becomes significantly more alkaline. These fluctuations are probably
related to cell damage during the injection and further wound repair.
After 20 h pH of interstitial fluid was equal to 7,65, which is in
between previously measured values and close to the blood pH known for
other fishes.
Key
words: Danio rerio, encapsulated optical sensors,
in vivo measurements, physiological pH
