Journal of Stress Physiology & Biochemistry, Vol. 12 No. 2 2016, pp. 39-52 ISSN 1997-0838
Original Text Copyright (cc) 2016 by  Deryabin and Trunova

Full text in PDF Download to Citation Manager Permanent urlData source :  Google Scholar
QueryDate : 2016-12-24
Cites : 0

The Physiological and Biochemical Mechanisms Providing the Increased Constitutive Cold Resistance in the Potato Plants, Expressing the Yeast SUC2 Gene Encoding Apoplastic Invertase

A.N. Deryabin, Т.I. Trunova

  K.A. Timiryazev Institute of Plant Physiology of the Russian Academy of Sciences,  Botanicheskaya street 35, Moscow, 127276, Russia


Received April 18, 2016

The expression of heterologous genes in plants is an effective method to improve our understanding of plant resistance mechanisms. The purpose of this work was to investigate the involvement of cell-wall invertase and apoplastic sugars into constitutive cold resistance of potato (Solanum tuberosum L., cv. Désirée) plants, which expressed the yeast SUC2 gene encoding apoplastic invertase. WT-plants of a potato served as the control. The increase in the essential cell-wall invertase activity in the leaves of transformed plants indicates significant changes in the cellular carbohydrate metabolism and regulatory function of this enzyme. The activity of yeast invertase changed the composition of intracellular sugars in the leaves of the transformed potato plant. The total content of sugars (sucrose,  glucose, fructose) in the leaves and apoplast was higher in the transformants, in comparison by WT-plants. Our data indicate higher constitutive resistance of transformants to severe hypothermia conditions compared to WT-plants. This fact allows us to consider cell-wall invertase as a enzyme of carbohydrate metabolism playing an important regulatory role in the metabolic signaling upon forming increased plant resistance to low temperature. Thus, the potato line with the integrated SUC2 gene is a convenient tool to study the role of the apoplastic invertase and the products of its activity during growth, development and formation constitutive resistance to hypothermia.

Key words:    Saccharomyces cerevisiae, Solanum tuberosum, invertase, low temperature, sugars, transformed plants

Back to issue content
интернет статистика
Free blog