Injections of Encapsulated pH Sensor SNARF-1 do not Induce Apparent Stress Reaction in Larvae of Endemic Baikal Caddisflies Baicalina thamastoides

Gurkov, A.N.; Belousova, I.A.; Shchapova, E.P.; Baduev, B.K.; Vereshchagina, K.P.; Timofeyev, M.A.

Journal of Stress Physiology & Biochemistry, Vol. 12 No. 1 2016, pp. 52-59 ISSN 1997-0838
Original Text Copyright (cc) 2015 by Gurkov, Belousova, Shchapova, Baduev, Vereshchagina and Timofeyev

ORIGINAL ARTICLE

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QueryDate : 2016-12-24
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Injections of Encapsulated pH Sensor SNARF-1 do not Induce Apparent Stress Reaction in Larvae of Endemic Baikal Caddisflies Baicalina thamastoides

A.N. Gurkov¹*, I.A. Belousova^1,2, E.P. Shchapova¹, B.K. Baduev¹, K.P. Vereshchagina¹, M.A. Timofeyev¹

¹ Institute of Biology, Irkutsk State University, 664003, Irkutsk, Karl Marx str., 1, Russia
² Institute of Systematics and Ecology of Animals, Siberian Branch of the Russian Academy of Sciences, 630091, Novosibirsk, Frunze str. 11, Russia

*E-Mail: a.n.gurkov@gmail.com

Received November 27, 2015

In the present study possibility of stress reaction of larvae of caddisflies Baicalina thamastoides Martynov, 1914 to injection of encapsulated sensors has been assessed. Activities of superoxide dismutase, non-specific esterases and glutathione S-transferase, which play important roles in functioning of organism defense systems, were used as biomarkers. Enzyme activities were measured after injections of empty microcapsules and microcapsules with fluorescent pH sensor SNARF-1, as well as after inlections of physiological saline solution and solution of the fluorescent sensor. Obtained results argue for nontoxicity and no apparent stress reaction of antioxidant and detoxification systems of B. thamastoides larvae to both types of microcapsules.

Key words: antioxidant defense, Baikal, microcapsules, Trichoptera