TY - JOUR AU - Dhamgaye, S. AU - Gadre, R. T1 - Salinity Stress Effects on Growth and Nitrate Assimilation in Bean Seedlings Likely to be Mediated via Nitric Oxide JO - Journal of Stress Physiology & Biochemistry Y1 - 2015 VL - 11 IS - 4 SP - 137 EP - 146 UR - http://www.jspb.ru/issues/2015/N4/JSPB_2015_4_137-146.pdf KW - NaCl effects KW - Phaseolus vulgaris KW - nitrate reductase KW - nitrate assimilation KW - nitric oxide stress N1 - Owner: Алексей N1 - Added to JabRef: 2015.12.02 N2 - Background : Salinity stress usually imposes adverse effects on plant systems, but the severity depends upon plant species, growth status and genotype, nutritional and environmental conditions etc. Present study analyses salinity effects on growth and in vivo nitrate reductase activity (NRA) in Phaseolus vulgaris seedlings to work out the mechanism.

Results : Supply of 10-200 mM NaCl with 10 mM KNO3 for 24 h in continuous light reduced the overall growth of the bean seedlings, with perfect –ve correlation for seedling weight, root length and shoot length. Salinity effect with 10 mM NH4Cl was lesser and with 10 mM NH4NO3 was intermediary. NaCl treatment with 10 mM KNO3 reduced the fresh wt of the root as well as shoot tissue but increased in vivo NRA exerting strong correlation and more prominent effect in the root tissue. Very high NaCl concentration prominently increased NRA at 1, 10 and 50 mM KNO3 showing inverse gradation in effect. Salt treatment with NH4NO3, reduced the in vivo NRA of the root tissue, but the stress parameters, like proline and peroxidase were increased.

Conclusions : The salinity stress effects on NRA are less severe with NH4+, more prominent for root and more effective at low NO3- concentration. Inverse correlation between decrease in fresh mass and increase in NRA with salinity suggests the involvement of NR in the synthesis of nitric oxide and the observed effect of stress is the balance of two factors reduced assimilatory effect and increased nitric oxide stress. ER -