TY - JOUR AU - Kolesnikova, E.V. AU - Ozolina, N.V. AU - Nurminsky, V.N. AU - Nesterkina, I.S. AU - Sitneva, L.A. AU - Lapteva, T.I. T1 - Evaluation of the Effect of Oxidative Stress on Roots of Red Beet (Beta vulgaris L.) JO - Journal of Stress Physiology & Biochemistry Y1 - 2014 VL - 10 IS - 4 SP - 5 EP - 12 UR - http://www.jspb.ru/issues/2014/N4/JSPB_2014_4_05-12.pdf KW - enzyme activity KW - electrolyte outflow KW - root of red beet KW - oxidative stress KW - stability of vacuoles N1 - Owner: Алексей N1 - Added to JabRef: 2014.11.12 N2 - In the present investigation it was studied the effect of oxidative stress on root of red beet. The degree of stress exposure was assessed at various levels: in tissues of roots (conductometric method), on isolated vacuoles (time-lapse video recording), and transport activity of the vacuolar membrane enzyme, namely V-H+-ATPase (fluorescent probe method). The obtained results allow to conclude the negative effects of oxidative stress on different levels of plant organization. There were several effects: a significant increase of outflow of electrolytes from the tissues of roots, a great decrease of half-life time of isolated vacuoles, and a 2 times reduction of transport activity of tonoplast H+-ATPase. Thus, the methods used in this study can be applied to assess the intensity of the impact of oxidative stress on plants. ER -