Journal of Stress Physiology & Biochemistry, Vol. 9 No. 4 2013, pp. 65-72 ISSN 1997-0838
Original Text Copyright (cc) 2013 by    Yadollhhi Nooshabadi, Mashayekhi



ORIGINAL ARTICLE
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Enzyme activity and seedling growth of soybean seeds under accelerated aging

Yadollhhi Nooshabadi S.J.1*, Mashayekhi F.2

1  Department of Agronomy and Plant Breeding, Faculty of Agri, Iran.
2 Department of Soil Science, University of Shahed, Tehran, Iran.


*E-Mail:  fatherofseed@gmail.com


Received May 25, 2013


Seed aging is the main problem of seed storage.  Changes of bio-chemical and  reduction of seedling growth are consequence of seed deterioration. An experiment was conducted to evaluate the effects of accelerated aging on soybean seed germination indexes and enzyme activity.  Seeds were incubated in closed plastic boxes for the accelerated aging treatments. Three accelerate aging regimes were performed by placing seeds at 41°C and relative humidity (RH) of 90-100 % for 0, 2, 4, 6 and 8 days periods.  Our results showed that increasing aging duration  resulted  higher  reduction  in  germination  characteristics,  catalase  and  ascorbate peroxidase. Germination percentage, means time to germination, germination index, normal seedling percentage and enzyme activity decrease significantly.

Key words:   Germination characteristics, Enzyme activity, Soybean seed, Aging

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