Journal of Stress Physiology & Biochemistry, Vol. 9 No. 4 2013, pp. 345-351 ISSN 1997-0838
Original Text Copyright (cc) 2013 by Madyarova, Lubyaga, Gurkov, Vereshchagina, Kondrat’eva, Shchapova, Timofeyev, Luckenbach, Bedulina

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A Comparative Analysis of hsp70 mRNA and HSP70 Protein Expression in Two Distant Populations of the Baikal Amphipod Eulimnogammarus  verrucosus (Gerstf., 1858)

Madyarova E.V.1, Lubyaga J.А.1, Gurkov А.N.1, Vereshchagina К.P.1, Kondrat’eva Е.М.1, Shchapova Е.P.1, Timofeyev М.А.1, Luckenbach T.2, Bedulina D.S.1

1 Irkutsk State University. 664003 Irkutsk, K. Marx str., 1, Russia
2 Helmholtz Centre for Environmental Research – UFZ, 04318 Leipzig, Permoser Straße 15, Germany.


Received August 20, 2013

Basal levels of  hsp70 (mRNA) and HSP70 (protein) were measured in representatives from two distant populations of the amphipod Eulimnogammarus verrucosus (Gerstf.) endemic to Lake Baikal using quantitative PCR (qPCR) and Western blotting upon lab acclimation. In addition, hsp70 mRNA and HSP70 protein levels in animals subjected to gradual temperature increase were measured. In contrast to  hsp70 mRNA levels that were similar in both populations basal HSP70 protein levels were  higher  in  representatives  of  the  southern  population  than  compared  to  the  northern population.  Changes  in  hsp70 mRNA  levels  at  increased  temperature  tended  to  be  more pronounced in the southern population compared to the northern one, whereas HSP70 protein changed only slightly in both populations. The data indicate differences in the phenotypic plasticity of the hsp/HSP70 stress response in different populations of this species.

Key words:  Amphipods, Baikal, Eulimnogammarus verrucosus, gene expression, hsp70

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