Journal of Stress Physiology &
Biochemistry, Vol. 9 No. 4
2013, pp. 345-351 ISSN 1997-0838
Original Text Copyright (cc) 2013 by Madyarova, Lubyaga, Gurkov,
Vereshchagina, Kondrat’eva, Shchapova, Timofeyev, Luckenbach, Bedulina
ORIGINAL ARTICLE
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QueryDate : 2016-12-24
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A Comparative Analysis of hsp70 mRNA and HSP70 Protein
Expression in Two Distant Populations of the Baikal Amphipod Eulimnogammarusverrucosus (Gerstf., 1858)
1 Irkutsk State University.
664003 Irkutsk, K. Marx str., 1, Russia 2 Helmholtz Centre for Environmental Research – UFZ, 04318
Leipzig, Permoser Straße 15, Germany.
*E-Mail: m.a.timofeyev@gmail.com
Received August 20, 2013
Basal levels of hsp70 (mRNA) and HSP70 (protein)
were measured in representatives from two distant populations of the
amphipod Eulimnogammarus verrucosus
(Gerstf.) endemic to Lake Baikal using quantitative PCR (qPCR) and
Western blotting upon lab acclimation. In addition, hsp70 mRNA and HSP70 protein levels
in animals subjected to gradual temperature increase were measured. In
contrast to hsp70 mRNA
levels that were similar in both populations basal HSP70 protein levels
were higher in representatives of
the southern population than compared
to the northern population. Changes in
hsp70 mRNA levels at increased
temperature tended to be more pronounced in the
southern population compared to the northern one, whereas HSP70 protein
changed only slightly in both populations. The data indicate
differences in the phenotypic plasticity of the hsp/HSP70 stress
response in different populations of this species.
