Journal of Stress Physiology & Biochemistry, Vol. 9 No. 4 2013, pp. 32-38 ISSN 1997-0838
Original Text Copyright (cc) 2013 by    Tabatabaei



ORIGINAL ARTICLE
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Effect of Salicylic Acid and Ascorbic Acid on Germination Indexes and Enzyme Activity of Sorghum Seeds under Drought Stress

Tabatabaei S. A.

  Agricultural and Natural Resources Research Center of Yazd, Iran

*E-Mail:  Omid0091@yahoo.com


Received May 18, 2013


Seed priming methods have been used to increase germination characteristics under stress conditions. The effects of drought stress (0, -4, -8, -12 and -16 bar) and salicylic acid 25 ppm at 15 °C for  15  h  and  ascorbic  acid  25 ppm  at  15 °C for 15  h on  germination  percentage, germination  index,  means  time  to  germination,  normal  seedling  percentage  and  enzyme activity were  assessed  in  the  laboratory  for  sorghum  seeds  (Sorghum  bicolor  L.).  Results showed  that  the  highest  germination  percentage  (83.33%),  normal  seedling  percentage (69.67%), germination index (25.29) and the minimum means time to germination (2.87) were attained  from  priming  with  salicylic  acid  in  control  conditions.  Therefore,  seed  priming significantly (p≤ 0.01) increased germination characteristics as compared to the unprimed under drought stress. Also, priming increased catalase and ascorbate peroxidase as compared to the unprimed seeds.

Key words:    Sorghum  bicolor  L.  Hormone-priming,  Germination  characteristics,  Drought  stress, Enzyme activity

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