TY  - JOUR
AU  - Perveen, Salma
AU  - Shinwari, Kamran Iqbal
AU  - Jan, Mehmood
AU  - Malook, Ijaz
AU  - Rehman, Shafiq
AU  - Ali Khan, Murad
AU  - Muhammad, Jamil
T1  - Low Temperature Stress Induced Changes in Biochemical Parameters, Protein Banding Pattern and Expression of Zat12 and Myb Genes in Rice Seedling
JO  - Journal of Stress Physiology & Biochemistry
Y1  - 2013
VL  - 9
IS  - 4
SP  - 193
EP  - 206
UR  - http://www.jspb.ru/issues/2013/N4/JSPB_2013_4_193-206.pdf

KW  - low temperature
KW  - biochemical parameters
KW  - protein
KW  - gene expression
KW  - Oryza sativa L.




N1  - Owner: Алексей
N1  - Added to JabRef: 2013.10.14
N2  - Low temperature stress is one of the main abiotic factors that reduce the productivity of many crops in hilly areas around the world. In this study, rice seedling were exposed to low temperature stress (control, 0°C, -2°C, -4°C and -6°C) for 2 hr to observe its effect on two rice varieties (Basmati-385 and Shaheen Basmati) through ion and proline contents, photosynthetic pigments, total protein content,  protein  banding  pattern  and  expression  of  Zat12  and  Myb  genes.  Resulted  showed different patterns of accumulation of Na+ K+ and Ca+2 ions with the decrease in temperature in both varieties.  Proline  accumulation  was  gradually  increased  in  both  varieties  with  the  decrease  in temperature.  Photosynthetic  pigments  (Chlorophyll  (Chl)  a,  b  and  carotene)  were  negatively affected by low temperature stress in both varieties, however, carotene content was much affected than Chl a and b. Nonsignificant variation in protein contents was observed at all levels of low temperature, but the effects of low temperature stress on protein banding pattern of Basmti-385 and Shaheen Basmati were different at different treatments. RT-PCR results indicated that  ZAT12 was upregulated by short term low temperature stress while  OsMYB show slight upregulation at -2°C as compared to the other treatments. This study identified that ZAT12 and OsMYB function as a positive regulator to mediate tolerance of rice seedlings at low temperature stress.
ER  -