Journal of Stress Physiology & Biochemistry, Vol. 9 No. 3 2013, pp. 44-53 ISSN 1997-0838
Original Text Copyright (cc) 2013 by   Saleh



ORIGINAL ARTICLE
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Evaluation of some upland cotton (Gossypium hirsutum L.) varieties for salinity tolerance screening

Basel Saleh

Department of Molecular Biology and Biotechnology, AECS, P.O. Box 6091, Damascus, Syria


*E-Mail: ascientific@aec.org.sy


Received  February 5, 2013

This investigation aimed to evaluate five upland cotton  (Gossypium hirsutum L.)  (Aleppo118, Aleppo33/1, Aleppo90, Raqqa5, and Deir-Ezzor22)  varieties based on physiological indices and genetic variation using AFLP marker. These varieties  were evaluated under control and saline conditions (50, 100 & 200 mM NaCl) for 56 days. Various physiological indices were detected in this investigation. In this respect, Deir-Ezzor22 variety differed by showing high salt tolerance relative to the other tested varieties. Otherwise, PCR amplification with 7 AFLP PCs primer combinations revealed  that  Deir-Ezzor22  variety characterized by 15 unique positive markers compared to the other tested varieties. Based on physiological study and AFLP technique, it can be concluded that genetic variation detected by AFLP marker supported the physiological indices among the tested cotton varieties. These varieties present considerable interest forngenetic studies and plant improvement.

Key words:    AFLP marker, Cotton, Physiological parameters, Salt stress

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