TY - JOUR AU - Bennani, Kawtar T1 - Trifolium isthmocarpum Brot, a salt-tolerant wild leguminous forage crop in salt-affected soils JO - Journal of Stress Physiology & Biochemistry Y1 - 2013 VL - 9 IS - 3 SP - 299 EP - 317 UR - http://www.jspb.ru/issues/2013/N3/JSPB_2013_3_299-317.pdf KW - NaCl stress KW - Pasture crop KW - Salt-affected land KW - Trifolium isthmocarpum Brot KW - Wild Legumes N1 - Owner: Алексей N1 - Added to JabRef: 2013.07.17 N2 - Plant scientists are investigating the potential of previously unexploited legume species where environmental and biological stresses constrain the use of more conventional forage crops or where these species are better suited to the needs of sustainable agriculture. Trifolium isthmocarpum Brot., Moroccan clover, occurs as a weed in different habitats in Morocco. It grows in moderately saline areas, where traditional forage legumes cannot be cultivated; however, it has not been widely studied despite its good palatability. The salt tolerance was studied between natural field conditions and glasshouse. The extensive field studies have recorded the species in many different habitats ranging from healthy agricultural lands to abandoned saline areas. The plants maintained high nodulation capacity (ranging between 60% and 97%) and nitrogenase activities (average 2.04 µmol C2H4 plant-1 h-1) in different habitats. Shoot systems of plants collected from salt-affected soils exhibited higher concentrations of Na+ and Cl- than those collected from healthy soils. Greenhouse experiments showed that germination percentage and vigor value of the studied species was not significantly (P > 0.05) affected at 160 mM NaCl, and that 25% of the germination ability was maintained when growing on substrats containing 240 mM NaCl. The growth rate of seedlings was not signicantly affected by 160 mM NaCl but was reduced by 38% under 240 mM NaCl. Leaf succulence and indices of leaf water status did not differ among the salt treatments, whereas relative water content was reduced by only 8% and water content at saturation increased by about 12% at high salt concentrations in the growing medium. This study suggest recommending the cultivation of T. isthmocarpum in salt-affected soils, which are widespread and pose a problem for the farmers of Morocco and other countries in the world's arid belt. ER -