Journal of Stress Physiology & Biochemistry, Vol. 9 No. 3 2013, pp. 262-271 ISSN 1997-0838
Original Text Copyright (cc) 2013 by 
Jain, Mittal, Gadre

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QueryDate : 2016-12-24
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Effect of PEG-6000 Imposed Water Deficit on Chlorophyll Metabolism in Maize Leaves

Meeta Jain*, Mini Mittal and Rekha Gadre

School of Biochemistry, Devi Ahilya University, Takshashila Campus, Khandwa Road, Indore – 452 017, India.


Received  April  2
, 2013

Drought stress is one of the major abiotic constraint limiting plant growth and productivity world wide. The current study was undertaken with the aim to investigate the effect of water deficit imposed by PEG-6000, on chlorophyll metabolism in maize leaves to work out the mechanistic details. Leaf segments prepared from primary leaves of etiolated maize seedlings were treated with varying concentrations of polyethylene glycol-6000 (PEG-6000; w/v- 5%, 10%, 20%, 30%) in continuous light of intensity 40 Wm-2 at 262 C for 24 h in light chamber. The results demonstrate a concentration dependent decline in chlorophyll content with increasing concentration of polyethylene glycol-6000 (PEG-6000). Reduction in chlorophyll ‘a’ level was to a greater extent than the chlorophyll ‘b’. The RNA content decreased in a concentration dependent manner with PEG, however, proline content increased significantly. Relative water content decreased significantly with the supply of 30% PEG only. A substantial decrease in chlorophyll synthesis due to significant reduction in ALA content and ALAD activity, with no change in chlorophyllase activity with the supply of PEG suggests that water deficit affects chlorophyll formation rather than its degradation.

Key words:     Chlorophyll metabolism, PEG-6000, Zea mays

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