Journal of Stress Physiology & Biochemistry, Vol. 9 No. 3 2013, pp. 251-261 ISSN 1997-0838
Original Text Copyright (cc) 2013 by 
Oboznyi, Kolupaev, Vayner, Yastreb

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The Role of Superoxide Dismutase in Inducing of Wheat Seedlings Tolerance to Osmotic Shock

Oboznyi A.I., Kolupaev Yu.E.*, Vayner A.A., Yastreb T.O.

V.V. Dokuchaev Kharkiv National Agrarian University, p/o «Communist-1», Kharkiv, 62483, Ukraine


Received  March 30
, 2013

Influence of short-term hardening osmotic exposure (immersion in 1 M sucrose solution with subsequent transferring to distilled water for 20 min) on the hydrogen peroxide generation and superoxide dismutase activity in wheat (Triticum aestivum L., cv. Elegiya) seedlings and their tolerance to osmotic shock were investigated. During the initial 30 min after osmotic exposure, the increasing of hydrogen peroxide amount in roots and shoots (to a lesser extent) was observed, but the resistance of the seedlings and superoxide dismutase (SOD) activity decreased. Sometime later the decrease in hydrogen peroxide amount and the increase of seedlings tolerance to osmotic shock took place. SOD activity increased in 10 min after hardening osmotic exposure. Transient accumulation of hydrogen peroxide induced in this way was suppressed by the treatment of seedlings with sodium diethyldithiocarbamate (DDC), SOD inhibitor. DDC and hydrogen peroxide scavenger dimethylthiourea decreased positive hardening effect of osmotic exposure on the development of seedlings tolerance. It was concluded that SOD providing the generation of signal hydrogen peroxide pool took part in the induction of seedlings tolerance to osmotic shock development caused by preliminary hardening effect.

Key words:     reactive oxygen species, hydrogen peroxide, superoxide dismutase, hardening, osmotic shock

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