Journal of Stress Physiology & Biochemistry, Vol. 9 No. 3 2013, pp. 220-231 ISSN 1997-0838
Original Text Copyright (cc) 2013 by 
Nageshbabu, Usha, Jyothi, Sharadamma, Rai, Devaraj

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QueryDate : 2016-12-24
Cites : 3

Expression of miRNAs confers enhanced tolerance to drought and salt stress in Finger millet (Eleusine coracona)

Nageshbabu R.1*, Usha1, Jyothi M.N.1, Sharadamma N.2, Rai D.V.3 and Devaraj V.R.4

1 Post Graduate Department of Biochemistry, Maharani’s Science College for Women, Bangalore-560001 India
2 Department of Biochemistry, Indian Institute of Science, Bangalore -560012 India
3 Centre for Bioinformatics, Faculty of Biological Engineering, Shobhit University, Meerut, India
4 Department of Biochemistry, Central College Campus, Bangalore University, Bangalore -560001 India


Received  March
19, 2013

Plants respond to the environmental cues in various ways, recent knowledge of RNA interference in conferring stress tolerance had become a new hope of developing tolerant varieties. Here we attempt to unfold the molecular mechanism of stress tolerance through miRNA profiling and expression analysis in Finger millet (Eleusine coracona) under salt and drought stress conditions. The expression analysis of 12 stress specific conserved miRNAs was studied using semi-quantitative real time PCR and Northern blot assay. Our studies revealed that, although most of the miRNAs responded to the stresses, the expression of particular miRNA differed with the nature of stress and the tissue. The expression analysis was correlated with the existing data of their target genes. Abiotic stress up-regulated miRNAs are expected to target negative regulators of stress responses or positive regulators of processes that are inhibited by stresses. On the other hand, stress down-regulated miRNAs may repress the expression of positive regulators and/or stress up-regulated genes. Thus the current study of miRNAs and their targets under abiotic stress conditions displays miRNAs may be good candidates to attribute the stress tolerance in plants by transgenic technology.

Key words:     Drought; Finger millet; miRNA; Salt stress

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