Journal of Stress Physiology & Biochemistry, Vol. 9 No. 3 2013, pp. 139-151 ISSN 1997-0838
Original Text Copyright (cc) 2013 by  Singh, Sharma



ORIGINAL ARTICLE
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Antioxidative Response of Various Cultivars of Sorghum (Sorghum bicolor L.) to Drought Stress

Gurpreet Singh* and Nidhi Sharma

Post-Graduate Department of Biotechnology, Lyallpur Khalsa College, GT Road, Jalandhar -144001 Punjab (INDIA)


*E-Mail: s_gp@rediffmail.com


Received  February 26, 2013

The present study was conducted with the aim to identify the response of antioxidant enzyme activities in seedlings of different sorghum cultivars under mannitol stress. Seven-day old seedlings were subjected to 100-500 mM mannitol stress which resulted in the decreases in shoot/root length and relative water content thus indicating the primary response to these tissues at phenotypic level. The level of lipid peroxidation as well as the specific activity of antioxidant enzymes such as peroxidase, catalase and superoxide dismutase increased at higher conc. except at 200 mM conditions. The level of catalase and peroxidase decreased at 500 mM conc. In the two different cultivars whereas the activity of superoxide dismutase consistently increased in response to the mannitol stress. Our data demonstrate that drought responsiveness tolerance in sorghum cultivars during germination is associated with enhanced activity of antioxidant enzymes.

Key words:    Catalase, drought stress, peroxidase, relative water content, superoxide dismutase, sorghum bicolor. 

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