Journal of Stress Physiology & Biochemistry, Vol. 9 No. 2 2013, pp. 219-231 ISSN 1997-0838
Original Text Copyright (cc) 2013 by   Narra, Regatte and Kodimyala



ORIGINAL ARTICLE
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Influence of Chlorpyrifos Stress on Protein Metabolism of Edible Crab Barytelphusa guerini, and its Recovery

Madhusudan Reddy Narra*, Rudra Reddy Regatte, Rajender Kodimyala

Department of Zoology, College of Science, Osmania University, Hyderabad 500 007, A.P. India

*E-Mail: narramadhusudan@gmail.com

Received December 28, 2012

The sublethal stress of chlorpyrifos on important metabolites and enzymes of protein metabolism was investigated in most important tissues (gills, muscle, hepatopancreas and nervous tissue) of freshwater edible crab, Barytelphusa guerini. The crabs were exposed to 0.07 mg/l (1/3 of LC50) for 7, 14, 21 and 28 days. After 28 days of exposure, they were released into fresh water and kept in the same for 21 days in order to study recovery (3days interval). Total protein content was decreased where as amino acids and ammonia was increased. After cessation of intoxication recovery was observed. Urea content was decrease in all tissues and glutamine exhibited mixed response and recovery was highest in muscle. The activities of protease and aminotransferases were elevated in tissues for 28 days. Recovery of these enzymes activities was noticed during depuration. Acid phosphatase activity was inhibited in hepatopancreas and nervous ganglion and induced in gills and muscle. Alkaline phosphatase activity was enhanced in gills and hepatopancreas and inhibited in muscle and nervous ganglion. All these enzymes showed recovery after released of crabs into fresh water.


Key words:   Barytelphusa guerini, Chlorpyrifos, Enzymes, Protein

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