TY - JOUR AB - Physiological and biochemical changes as well as the activities of anti-oxidative enzymes under lead (Pb2+) phytotoxicity were investigated in 20 days old soybean (Glycine max L.) seedlings grown hydroponically in the laboratory under different pH conditions. The rapid uptake of Pb 2+ was observed immediately after the start of treatment. The quantity of accumulation of Pb2+ was much higher in roots than in shoots, its level rising with increasing pH from 3.0 to 8.0 . Not only that, an oxidative stress conditions were observed due to increased level of superoxide anion radical and hydrogen peroxide in shoots and root cells of 20 days old seedlings when treated with Pb(NO3)2 at a concentration of 0, 500, 1000 and 2000 μM. Spectrometric assays of seedlings showed increased level of activities of antioxidant enzymes like catalase, peroxidase and glutathione reductase. The presence of thiobarbituric acid reacting substances (TBARS) indicates the enhanced lipid peroxidation compared to controls. The alteration in the activities of the antioxidant enzymes and the induction of lipid peroxidation reflects the presence of Pb2+, which may cause oxidative stress. AU - Mishra, Pankaj Kishor AU - Kumar, Umesh AU - Mishra, Meenakshi AU - Prakash, Veeru DA - 2013 ET - 2013 KW - Antioxidant enzymes, Catalase, Free radicals, Lipid peroxidation, oxidative stress, Superoxide dismutase M1 - 2 M3 - ORIGINAL ARTICLE PY - 2013 SE - 131 SN - 1997-0838 SP - 131-147 ST - Antioxidative Defense Responses to lead-induced Oxidative Stress in Glycine max L. CV. Merrill grown in Different pH Gradient T2 - Journal of Stress Physiology & Biochemistry TI - Antioxidative Defense Responses to lead-induced Oxidative Stress in Glycine max L. CV. Merrill grown in Different pH Gradient UR - http://www.jspb.ru/issues/2013/N2/JSPB_2013_2_131-147.pdf VL - 9 ID - 11 ER -