Journal of Stress Physiology &
Biochemistry, Vol. 9 No. 1
2013, pp. 289-295 ISSN 1997-0838
Original Text Copyright (cc) 2013 by Ozolina, Nesterkina,
Sitneva, Lapteva, Nurminsky, Kolesnikova and Salyaev
ORIGINAL
ARTICLE
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QueryDate : 2016-12-24
Cites : 0
Alteration of SH-group
Contents in Red Beet Roots and Vacuoles under Osmotic Stress
N.V. Ozolina, I.S. Nesterkina, L.A.
Sitneva, T.I. Lapteva, V.N. Nurminsky, E.V. Kolesnikova, R.K. Salyaev
Siberian Institute of Plant Physiology and Biochemistry SB RAS,
Irkutsk, Russia
*E-Mail: ozol@sifibr.irk.ru
Received October 30, 2012
The content of
sulfhydryl groups in homogenate of red beets and isolated vacuoles
under the conditions of osmotic stress was determined. It was
demonstrated that the common content of sulfhydryl groups in the
isolated vacuoles was 2.4 times higher, than in the homogenate. Under
the conditions of osmotic stress, it was primarily denoted the
reduction of common content of sulfhydryl groups in homogenate and in
the isolated vacuoles. The most interesting results were obtained in
determination of correlations between protein and non-protein
SH-groups. Under the conditions of osmotic stress, while the contents
of non-protein SH-groups in the isolated vacuoles was reduced,
non-protein SH-groups in homogenate was greatly increased. This may be
explained by the influx of the substances containing SH-groups out of
vacuoles. Obtained results allow us to conclude that vacuoles play an
important role in plant cell antioxidant processes and in maintenance
of intracellular redox-homeostasis.
Key
words: vacuoles; red beet roots; osmotic stress; sulfhydryl groups
