Glutathione is not involved in light-, Dark-, Ca- and H2O2- induced stomatal movement in Arabidopsis

Sarwar Jahan, Md; Bin Che Lah, M. K.; Bin Nordin, M. N.; Syed Kamarulzaman, S. S. B.

Journal of Stress Physiology & Biochemistry, Vol. 8 No. 3 2012, pp. 240-246 ISSN 1997-0838
Original Text Copyright (cc) 2012 by Jahan, Khairi Bin Che Lah, Nozulaidi Bin Nordin, and Syed Kamarulzaman

ORIGINAL ARTICLE

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QueryDate : 2016-12-24
Cites : 2

Glutathione is not involved in light-, Dark-, Ca- and H₂O₂- induced stomatal movement in Arabidopsis

Md Sarwar Jahan*, Mohd Khairi Bin Che Lah, Mohd Nozulaidi Bin Nordin, Syed S.B. Syed Kamarulzaman

Faculty of Agriculture and Biotechnology, Universiti Sultan Zainal Abidin, 20400 Kuala Terengganu, Malaysia
Tel: ++6-09-666-0241; Fax: ++6-09-666-0244
* E-mail: sarwarjahan@unisza.edu.my
Received June 15 2012

Glutathione (GSH), is a thiol-containing tripeptide, maintains redox homeostasis in plants under normal and stressful conditions. In this study, we investigated whether GSH involved in light-, dark-, Ca- and H₂O₂-induced stomatal movement in Arabidopsis. Application of GSH and a GSH decreasing chemical (CDNB; 1-chloro-2,4-dinitrobenzene) did not affect stomatal aperture in guard cells of Arabidopsis. Dark induced stomatal closure and light induced stomatal opening but pre-treatment of GSH and CDNB did not alter dark- and light-induced stomatal aperture. Treatment of guard cells with Ca and H₂O₂ did not affect GSH contents in guard cells but induced stomatal closure in both wild type and chorinal-1 (ch1-1) mutant plants. In addition, pre-treatment of GSH and CDNB did not affect Ca and H₂O₂-induced stomatal closure in both plants. Taken together these results suggest that GSH might not directly affect light-, dark-, Ca- and H₂O₂-induced stomatal movement in guard cells of Arabidopsis.

Key words: abscisic acid; monochlorobimane; stress