Journal of Stress Physiology & Biochemistry, Vol. 8 No. 3 2012, pp. 234-239 ISSN 1997-0838
Original Text Copyright (cc) 2012 by Jorjandi and Sharifi Sirchi



ORIGINAL ARTICLE
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The Effect of Priming on Germination and Seedling Growth of Alfalfa (Medicago sativa L.) under Salinity Stress

Jorjandi M.* and G.R. Sharifi Sirchi

Department of Agricultural Biotechnology, College of Agriculture, Shahid Bahonar University of Kerman, Iran.
Tel(uni) : 0098-341-3202638
* E-mail: mahsa.jorjandi@gmail.com

Received June 10 2012

Salinity stress is an abiotic stress which has harmful effects on germination, growth and yield of alfalfa (Medicago sativa L.) in many parts of the world. Seed priming is a way of increasing salt tolerance of plants. In this study, the effect of seed priming on germination and growth of alfalfa seedlings, Bami cultivar, under salinity condition was investigated. According to germination percentage of seeds under In vitro conditions, salicylic acid, Vitamin B12 and distilled water were selected as primes under salinities of 0, 5 and 10 ds.m-2 NaCl solution for further studies. These studies were carried out in a factorial experiment based on Completely Randomized Design (CRD) with three replications in greenhouse under controlled condition (25°C in day/night). Parameters like percentage and rate of germination, dry and fresh weight of seedling and leaf number were measured. Based on our research All of the treatments (priming, salinity and the interaction of priming with salinity) significantly (p<0.01) affected Hypocotyl length and radicle length and in low level of salt (0 ds.m2) germination and seedling growth indexes enhanced and also with applying salicylic acid both hypocotyl and radicle lengths improved.

Key words: salinity stress/ hydropriming/ alfalfa/ salicylic acid and Vitamin B12

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