Agrobacterium-mediated transformation of Nicotiana tabacum by disarmed strain At 699 resulted in considerable raising of growth and development of transgenic plants

Maximova, L. A.; Nurminskaya, J. V.; Kopytina, T. V.; Enikeev, A. G.

Journal of Stress Physiology & Biochemistry, Vol. 8 No. 1 2012, pp. 138-148 ISSN 1997-0838
Original Text Copyright (cc) 2012 by Maximova, Nurminskaya, Kopytina and Enikeev

ORIGINAL ARTICLE

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QueryDate : 2016-12-24
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Agrobacterium-mediated transformation of Nicotiana tabacum by disarmed strain At 699 resulted in considerable raising of growth and development of transgenic plants

Maximova L.A.*, Nurminskaya J.V., Kopytina T.V., and Enikeev A.G.

Siberian Institute of Plant Physiology and Biochemistry; SB RAS 664033, Irkutsk, post box 317, Russia
*E mail: lasema@sifibr.irk.ru

Received December 14, 2011

The question how long transgenesis invected alterations are demonstrated in a succeeding generations remains of great interest. In this study we describe the development of T₁-T₅ generations of Nicotiana tabacum L. transformed by Agrobacterium tumefacience strain 699 with disarmed plasmid. Tobacco plants were grown in the same environmental conditions. The characteristics of vegetative and generated parts had been assessed. Transgenic plants were superior to normal ones in leaf area and stem length, had earlier flowering and internodes development but not differ in a number and size of flowers. Growth activation is suggested to be a result of biotic stress induced by transformation.

Key words: Nicotiana tabacum, disarmed strain, transgenic plant, development, T1-T5 generations