Journal of Stress Physiology & Biochemistry, Vol. 2, No. 1, 2006, pp. 28-34.
Original Text Copyright (cc) 2006 by Svedentsov, Tumanova, Chtcheglova, Solomina



ORIGINAL ARTICLE 
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CONSERVATION LEUKOCYTES IN THE CONDITIONS OF CRYOANABIOSIS (-400С)

Svedentsov E.P., Chtcheglova O.O., Tumanova T.V., Solomina O.N

Laboratory cryophysiology of blood, Komi Institute of Physiology of Russian Academy of Sciences, 167982 Syktyvkar, Russia

Received November 3, 2005

Trying to meet the requirements in leukocytes the clinicists are facing the pressing problem of conservation of cells in a functionally valid condition which is possible in deep cryoanabiosis (-196°C). This conservation method is not, for the number of reasons, available for the purpose of a wide use. The aim of the present was development of an efficient, readily available and economical method for conservation of leukocytes at a moderately low temperature of -40°C. It was shown that use of this method allows for conservation of blood nuclear cells in a functionally valid condition for a period of 30 days.

Key words: leukocytes, cryoanabiosis, cryopreservative, exponential programme for freezing



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