Journal of Stress Physiology & Biochemistry, Vol. 1, No. 1, 2005, pp. 6-14.
Original Text Copyright (cc) 2005 by Afzal, Basra and Iqbal.



ORIGINAL ARTICLE 
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THE EFFECTS OF SEED SOAKING WITH PLANT GROWTH REGULATORS ON SEEDLING VIGOR OF WHEAT UNDER SALINITY STRESS.


Irfan Afzal*, Shahzad M.A. Basra* and Amir Iqbal**

*Department of crop physiology, University of Agriculture, Faisalabad, Pakistan;
** Monsanto Pakistan Agritech (Pvt.) Ltd., Multan Road Lahore, Pakistan

tel.: +92 300 9658671, fax: +92 41 9200183, e-mail: irita76@yahoo.com;

Received March 10, 2005
Received in revised form April 19, 2005


Effects of seed soaking with plant growth regulators (IAA, GA3, kinetin or prostart) on wheat (Triticum aestivum cv. Auqab-2000) emergence and seedling growth under normal (4 dS/cm) and saline (15 dS/cm) conditions were studied to determine their usefulness in increasing relative salt-tolerance. During emergence test, emergence percentage and mean emergence time (MET) were significantly affected by most of priming treatments, however, root and shoot length, fresh and dry weight of seedlings were significantly increased by 25 ppm kinetin followed by 1% prostart for 2 h treatments under both normal and saline conditions. All pre-sowing seed treatments decreased the electrolyte leakage of steep water as compared to that of non-primed seeds even after 12 h of soaking. Seed soaking with 25 ppm kinetin induced maximum decrease in electrolyte leakage while an increase in electrolyte leakage was observed by 25, 50 or 100 ppm IAA treatments. It is concluded that priming has reduced the severity of the effect of salinity but the amelioration was better due to 25 ppm kinetin and 1% prostart (2 h) treatments as these showed best results on seedling growth, fresh and dry weights under non-saline and saline conditions whereas seed soaking with IAA and GA3 were not effective in inducing salt tolerance under present experimental material and conditions.

Key words: seed treatments, plant growth regulators, salinity stress, seedling vigor, wheat seed



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